TY - T1 - Evaluating Lignins as Enzyme Substrates : Insights and Methodological Recommendations from a Study of Laccase-Catalyzed Lignin Polymerization SN - / UR - http://hdl.handle.net/10138/166362 T3 - A1 - West, Mark A.; Hickson, Aynsley C.; Mattinen, Maija-Liisa; Lloyd-Jones, Gareth A2 - PB - Y1 - 2014 LA - eng AB - Lignin preparations from kraft and sulfite pulping, steam explosion, and enzyme saccharification processes were assessed as substrates for lignin polymerization catalyzed by Trametes hirsuta laccase (ThL). Oxygen consumption associated with laccase catalyzed oxidation of the selected lignins was measured using a microplate-based oxygen assay. Laccase-induced changes in the molecular masses of the lignin polymers were assessed with aqueous-alkaline size exclusion chromatography (SEC) and changes ... VO - IS - SP - OP - KW - lignin; Laccase; Mediator; Polymerization; Ultrafiltration; Phenolic; Oxygen sensor; FUNGAL LACCASES; MOLECULAR-WEIGHT; STEAM EXPLOSION; KRAFT LIGNIN; TECHNICAL LIGNINS; BLACK LIQUOR; LIGNOSULFONATES; SOFTWOOD; PRETREATMENT; BIOREFINERY; 116 Chemical sciences N1 - PP - ER -