TY  -  
T1  - Expanding the feruloyl esterase gene family of Aspergillus niger by characterization of a feruloyl esterase, FaeC 
SN  -  /  
UR  - http://hdl.handle.net/10138/308036 
T3  -  
A1  - Dilokpimol, Adiphol; Mäkelä, Miia R.; Mansouri, Sadegh; Belova, Olga; Waterstraat, Martin; Bunzel, Mirko; de Vries, Ronald P.; Hilden, Kristiina S. 
A2  -  
PB  -  
Y1  - 2017 
LA  - eng 
AB  - A feruloyl esterase (FAE) from Aspergillus niger N402, FaeC was heterologously produced in Pichia pastoris X-33 in a yield of 10 mg/L. FaeC was most active at pH 7.0 and 50 degrees C, and showed broad substrate specificity and catalyzed the hydrolysis of methyl 3,4-dimethoxycinnamate, ethyl ferulate, methyl ferulate, methyl p-coumarate, ethyl coumarate, methyl sinapate, and methyl caffeate. The enzyme released both ferulic acid and p-coumaric acid from wheat arabinoxylan and sugar beet pectin (u... 
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IS  -  
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OP  -  
KW  - Aspergillus niger; Ferulic acid; Feruloyl esterase; Pectin; Xylan; SUGAR-BEET PULP; CELL-WALL POLYSACCHARIDES; ACID ESTERASES; DEGRADATION; ENZYME; CLASSIFICATION; PURIFICATION; BIOMASS; OLIGOSACCHARIDES; EXPRESSION; 1182 Biochemistry, cell and molecular biology 
N1  -   
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ER  -