TY  -  
T1  - Expression and purification of the extracellular domain of wild-type humanRET and the dimeric oncogenic mutant C634R 
SN  -  /  
UR  - http://hdl.handle.net/10138/332918 
T3  -  
A1  - Liu, Yixin; Ribeiro, Orquidea De Castro; Robinson, James; Goldman, Adrian 
A2  -  
PB  -  
Y1  - 2020 
LA  - eng 
AB  - The receptor tyrosine kinase RET is essential in a variety of cellular processes. RET gain-of-function is strongly associated with several cancers, notably multiple endocrine neoplasia type 2A (MEN 2A), while RET loss-of-function causes Hirschsprung's disease and Parkinson's disease. To investigate the activation mechanism of RET as well as to enable drug development, over-expressed recombinant protein is needed for in vitro functional and structural studies. By comparing insect and mammalian ce... 
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IS  -  
SP  -  
OP  -  
KW  - Receptor tyrosine kinase; Recombinant protein expression; Cysteine-rich domain; EPIDERMAL-GROWTH-FACTOR; PROTEIN N-GLYCOSYLATION; CYSTEINE-RICH DOMAIN; RET RECEPTOR; KINASE INHIBITORS; LIGAND; GDNF; MUTATIONS; BINDING; GDF15; 1182 Biochemistry, cell and molecular biology 
N1  -   
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ER  -