TY - T1 - Food-Like Growth Conditions Support Production of Active Vitamin B12 by Propionibacterium freudenreichii 2067 without DMBI, the Lower Ligand Base,or Cobalt Supplementation SN - / UR - http://hdl.handle.net/10138/183458 T3 - A1 - Deptula, Paulina; Chamlagain, Bhawani; Edelmann, Minnamari; Sangsuwan, Panchanit; Nyman, Tuula A.; Savijoki, Kirsi; Piironen, Vieno; Varmanen, Pekka A2 - PB - Y1 - 2017 LA - eng AB - Propionibacterium freudenreichii is a traditional dairy bacterium and a producer of short chain fatty acids (propionic and acetic acids) as well as vitamin B12. In food applications, it is a promising organism for in situ fortification with B12 vitamin since it is generally recognized as safe (GRAS) and it is able to synthesize biologically active form of the vitamin. In the present study, vitamin B12 and pseudovitamin biosynthesis by P. freudenreichii was monitored by UHPLC as a function of gro... VO - IS - SP - OP - KW - Propionibacterium freudenreichii; vitaminB12; cobalamin; pseudovitaminB12; food; 2D-PAGE; stress; LACTOBACILLUS-REUTERI CRL1098; LACTIC-ACID BACTERIA; DAIRY PROPIONIBACTERIA; SUBSP SHERMANII; METABOLISM; IDENTIFICATION; PROTEINS; LACTATE; SURVIVAL; B-12; 1183 Plant biology, microbiology, virology N1 - PP - ER -