TY - T1 - Molecular determinants of ER-Golgi contacts identified through a new FRET-FLIM system SN - / UR - http://hdl.handle.net/10138/305645 T3 - A1 - Venditti, Rossella; Rega, Laura Rita; Masone, Maria Chiara; Santoro, Michele; Polishchuk, Elena; Sarnataro, Daniela; Paladino, Simona; D'Auria, Sabato; Varriale, Antonio; Olkkonen, Vesa M.; Di Tullio, Giuseppe; Polishchuk, Roman; De Matteis, Maria Antonietta A2 - PB - Y1 - 2019 LA - eng AB - ER-TGN contact sites (ERTGoCS) have been visualized by electron microscopy, but their location in the crowded perinuclear area has hampered their analysis via optical microscopy as well as their mechanistic study. To overcome these limits we developed a FRET-based approach and screened several candidates to search for molecular determinants of the ERTGoCS. These included the ER membrane proteins VAPA and VAPB and lipid transfer proteins possessing dual (ER and TGN) targeting motifs that have bee... VO - IS - SP - OP - KW - ENDOPLASMIC-RETICULUM; PLASMA-MEMBRANE; LIPID-TRANSFER; BINDING; PHOSPHATIDYLSERINE; VAP; TRAFFICKING; TRANSPORT; PROTEINS; INTERACTOME; 3111 Biomedicine; 1182 Biochemistry, cell and molecular biology N1 - PP - ER -