TY  -  
T1  - Multiparametric Flow Cytometry Using Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes 
SN  -  /  
UR  - http://hdl.handle.net/10138/160336 
T3  -  
A1  - Telford, William G.; Shcherbakova, Daria M.; Buschke, David; Hawley, Teresa S.; Verkhusha, Vladislav V. 
A2  -  
PB  -  
Y1  - 2015 
LA  - eng 
AB  - Engineering of fluorescent proteins (FPs) has followed a trend of achieving longer fluorescence wavelengths, with the ultimate goal of producing proteins with both excitation and emission in the near-infrared (NIR) region of the spectrum. Flow cytometers are now almost universally equipped with red lasers, and can now be equipped with NIR lasers as well. Most red-shifted FPs of the GFP-like family are maximally excited by orange lasers (590 to 610 nm) not commonly found on cytometers. This has c... 
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IS  -  
SP  -  
OP  -  
KW  - IN-VIVO; RED; LASERS; YELLOW; ORANGE; GREEN; 3111 Biomedicine 
N1  -   
PP  -  
ER  -