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T1  - Multiplexed analysis of amino acids in mice brain microdialysis samples using isobaric labeling and liquid chromatography-high resolution tandem mass spectrometry 
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UR  - http://hdl.handle.net/10138/335776 
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A1  - Heininen, Juho; Julku, Ulrika; Myöhänen, Timo; Kotiaho, Tapio; Kostiainen, Risto 
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Y1  - 2021 
LA  - eng 
AB  - We developed a new multiplexed reversed phase liquid chromatography-high resolution tandem mass spectrometric (LC-MS/MS) method. The method is based on isobaric labeling with a tandem mass tag (TMT10-plex) and stable isotope-labeled internal standards, and was used to analyze amino acids in mouse brain microdialysis samples. The TMT10-plex labeling of amino acids allowed analysis of ten samples in one LC-MS/MS run, significantly increasing the sample throughput. The method provides good chromato... 
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KW  - Multiplexing; Isobaric labeling; Isotope dilution; Metabolites; Amino acids; High resolution tandem mass spectrometry; D-AMPHETAMINE; QUANTITATIVE PROTEOMICS; EXTRACELLULAR LEVELS; LC-MS; RAT; GLUTAMATE; METABOLITES; INCREASES; STRIATUM; GABA; 1182 Biochemistry, cell and molecular biology; 116 Chemical sciences 
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