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T1  - Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum 
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UR  - http://hdl.handle.net/10138/336378 
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A1  - Hafeez, Muhammad Nadeem; Khan, Mohsin Ahmad; Sarwar, Bilal; Hassan, Sameera; Ali, Qurban; Husnain, Tayyab; Rashid, Bushra 
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Y1  - 2021 
LA  - eng 
AB  - Gossypium arboreum is considered a rich source of stress-responsive genes and the EST database revealed that most of its genes are uncharacterized. The full-length Gossypium universal stress protein-2 (GUSP-2) gene (510 bp) was cloned in E. coli and Gossypium hirsutum, characterized and point mutated at three positions, 352-354, Lysine to proline (M1-usp-2) & 214-216, aspartic acid to serine (M2-usp-2) & 145-147, Lysine to Threonine (M3-usp-2) to study its role in abiotic stress tolerance. It wa... 
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KW  - WATER-DEFICIT STRESS; MEMBRANE THERMOSTABILITY; HETEROLOGOUS EXPRESSION; SALICORNIA-BRACHIATA; GOSSYPIUM-ARBOREUM; EXTREME HALOPHYTE; ESCHERICHIA-COLI; HIGH-TEMPERATURE; HEAT TOLERANCE; PROTEIN; 1182 Biochemistry, cell and molecular biology; 317 Pharmacy 
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