TY  -  
T1  - Palindromic sequence-targeted (PST) PCR, version 2: an advanced method for high-throughput targeted gene characterization and transposon display 
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UR  - http://hdl.handle.net/10138/331653 
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A1  - Kalendar, Ruslan; Shustov, Alexandr; Schulman, Alan 
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Y1  - 2021 
LA  - eng 
AB  - Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that exist in proximity to a known sequence tag, is currently predominantly based on PCR. Recently developed PCR-based methods allow for combining of sequence-specific primers with designed capturing primers capable of annealing to unknown DNA targets, which offer the rapidity and effectiveness of PCR. This study presents a methodological improvement to the previously described GW technique known as Palindromic Se... 
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KW  - 11831 Plant biology; genome walking; transposon display (TD); palindrome; restriction site; amplified fragment length polymorphism (AFLP); transposable elements (TE); biodiversity; ASYMMETRIC INTERLACED PCR; GENOME-WALKING METHOD; JAVA WEB TOOLS; IN-SILICO PCR; FAST PRIMER; AMPLIFICATION; FRAGMENTS; ELEMENTS; FASTPCR; REMAP 
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