TY  -  
T1  - Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking 
SN  -  /  
UR  - http://hdl.handle.net/10138/307667 
T3  -  
A1  - Kalendar, Ruslan; Shustov, Alexandr; Seppänen, Mervi Mirjam; Schulman, Alan Howard; Stoddard, Frederick Lothrop 
A2  -  
PB  -  
Y1  - 2019 
LA  - eng 
AB  - Genome walking (GW) refers to the capture and sequencing of unknown regions in a long DNA molecule that are adjacent to a region with a known sequence. A novel PCR-based method, palindromic sequence-targeted PCR (PST-PCR), was developed. PST-PCR is based on a distinctive design of walking primers and special thermal cycling conditions. The walking primers (PST primers) match palindromic sequences (PST sites) that are randomly distributed in natural DNA. The PST primers have palindromic sequences... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - 1183 Plant biology, microbiology, virology; ASYMMETRIC INTERLACED PCR; FAST PRIMER; AMPLIFICATION; FRAGMENTS; FASTPCR; TOOLS; PROBE 
N1  -   
PP  -  
ER  -