TY - T1 - Probing the Proton-Loading Site of Cytochrome C Oxidase Using Time-Resolved Fourier Transform Infrared Spectroscopy SN - / UR - http://hdl.handle.net/10138/318207 T3 - A1 - Gorbikova, Elena; Samsonov, Sergey A.; Kalendar, Ruslan A2 - PB - Multidisciplinary Digital Publishing Institute Y1 - 2020 LA - eng AB - Crystal structure analyses at atomic resolution and FTIR spectroscopic studies of cytochrome c oxidase have yet not revealed protonation or deprotonation of key sites of proton transfer in a time-resolved mode. Here, a sensitive technique to detect protolytic transitions is employed. In this work, probing a proton-loading site of cytochrome c oxidase from Paracoccus denitrificans with time-resolved Fourier transform infrared spectroscopy is presented for the first time. For this pu... VO - IS - SP - OP - KW - N1 - PP - ER -