TY  -  
T1  - Production of recombinant laccase from Coprinopsis cinerea and its effect in mediator promoted lignin oxidation at neutral pH 
SN  -  /  
UR  - http://hdl.handle.net/10138/336134 
T3  -  
A1  - Kontro, Jussi; Lyra, Christina; Koponen, Milla Jasmin; Kuuskeri, Jaana; Kähkönen, Mika; Wallenius, Janne; Wan, Xing; Sipilä, Jussi; Mäkelä, Miia; Nousiainen, Paula Annukka; Hilden, Kristiina 
A2  -  
PB  -  
Y1  - 2021 
LA  - eng 
AB  - Laccases are multi-copper oxidases that use molecular oxygen as the electron acceptor to oxidize phenolic and indirectly also non-phenolic substrates by mechanisms involving radicals. Due to their eco-friendliness and broad substrate specificity, laccases span a wide range of biotechnological applications. We have heterologously expressed a laccase from the coprophilic basidiomycete Coprinopsis cinerea (CcLcc9) in the methylotrophic yeast Pichia pastoris. The recombinant CcLcc9 (rCcLcc9) oxidize... 
VO  -  
IS  -  
SP  -  
OP  -  
KW  - 414 Agricultural biotechnology; 11832 Microbiology and virology; Coprinopsis cinerea; laccase characteristics; laccase-mediator systems; lignin depolymerization; structural analysis; FUNGAL LACCASES; MODEL COMPOUNDS; BOND-CLEAVAGE; METAL-IONS; DEPOLYMERIZATION; SYSTEMS; DESIGN 
N1  -   
PP  -  
ER  -