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T1  - Purification of Highly Active Alphavirus Replication Complexes Demonstrates Altered Fractionation of Multiple Cellular Membranes 
SN  -  /  
UR  - http://hdl.handle.net/10138/298263 
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A1  - Pietila, Maija K.; van Hemert, Martijn J.; Ahola, Tero 
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PB  -  
Y1  - 2018 
LA  - eng 
AB  - Positive-strand RNA viruses replicate their genomes in membrane-associated structures; alphaviruses and many other groups induce membrane invaginations called spherules. Here, we established a protocol to purify these membranous replication complexes (RCs) from cells infected with Semliki Forest virus (SFV). We isolated SFV spherules located on the plasma membrane and further purified them using two consecutive density gradients. This revealed that SFV infection strongly modifies cellular membra... 
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KW  - alphavirus; Semliki Forest virus; replication complex; purification; RNA replication; membrane fractionation; SEMLIKI-FOREST-VIRUS; VIRAL-RNA REPLICATION; SINDBIS VIRUS; IN-VITRO; CHIKUNGUNYA VIRUS; NONSTRUCTURAL POLYPROTEINS; ENDOPLASMIC-RETICULUM; ARBOVIRUS INFECTION; PLASMA-MEMBRANE; MINUS-STRAND; 1183 Plant biology, microbiology, virology 
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