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T1  - RNA-dependent RNA polymerase from heterobasidion RNA virus 6 is an active replicase in vitro 
SN  -  /  
UR  - http://hdl.handle.net/10138/334913 
T3  -  
A1  - Levanova, Alesia A.; Vainio, Eeva J.; Hantula, Jarkko; Poranen, Minna M. 
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Y1  - 2021 
LA  - eng 
AB  - Heterobasidion RNA virus 6 (HetRV6) is a double-stranded (ds)RNA mycovirus and a member of the recently established genus Orthocurvulavirus within the family Orthocurvulaviridae. The purpose of the study was to determine the biochemical requirements for RNA synthesis catalyzed by HetRV6 RNA-dependent RNA polymerase (RdRp). HetRV6 RdRp was expressed in Escherichia coli and isolated to near homogeneity using liquid chromatography. The enzyme activities were studied in vitro using radiolabeled UTP.... 
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KW  - 1182 Biochemistry, cell and molecular biology; DsRNA virus; Mycovirus; Replicase activity; Semi-conservative transcription; TNTase activity; Viral RdRp; DOUBLE-STRANDED-RNA; MINUS-STRAND; PROTEIN; MECHANISM; viral RdRp; dsRNA virus; DE-NOVO INITIATION; BACTERIOPHAGE-PHI-6; mycovirus; semi-conservative transcription; ROTAVIRUS VP1; MESSENGER-RNA; STRUCTURAL BASIS; replicase activity; TRANSFERASE-ACTIVITY 
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