TY - T1 - Regulation of INF2-mediated actin polymerization through site-specific lysine acetylation of actin itself SN - / UR - http://hdl.handle.net/10138/317249 T3 - A1 - A, Mu; Fung, Tak Shun; Francomacaro, Lisa M.; Huynh, Thao; Kotila, Tommi; Svindrych, Zdenek; Higgs, Henry N. A2 - PB - Y1 - 2020 LA - eng AB - INF2 is a formin protein that accelerates actin polymerization. A common mechanism for formin regulation is autoinhibition, through interaction between the N-terminal diaphanous inhibitory domain (DID) and C-terminal diaphanous autoregulatory domain (DAD). We recently showed that INF2 uses a variant of this mechanism that we term "facilitated autoinhibition," whereby a complex consisting of cyclase-associated protein (CAP) bound to lysine-acetylated actin (KAc-actin) is required for INF2 inhibit... VO - IS - SP - OP - KW - ADP-ACTIN; COFILIN; CYCLASE-ASSOCIATED PROTEIN; INF2; INVERTED FORMIN 2; LEVEL; MECHANISM; MONOMERS; NUCLEATION; U2OS; WH2 DOMAIN; WH2 motif; cyclase-associated protein; mitochondria; nucleation; 1182 Biochemistry, cell and molecular biology N1 - PP - ER -