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T1  - Single-molecule measurements of viral ssRNA packaging 
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UR  - http://hdl.handle.net/10138/239660 
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A1  - Hanhijarvi, Kalle J.; Ziedaite, Gabija; Bamford, Dennis H.; Haeggstrom, Edward; Poranen, Minna M. 
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Y1  - 2017 
LA  - eng 
AB  - Genome packaging of double-stranded RNA (dsRNA) phages has been widely studied using biochemical and molecular biology methods. We adapted the existing in vitro packaging system of one such phage for single-molecule experimentation. To our knowledge, this is the first attempt to study the details of viral RNA packaging using optical tweezers. Pseudomonas phage phi 6 is a dsRNA virus with a tripartite genome. Positive-sense (+) single-stranded RNA (ssRNA) genome precursors are packaged into a pre... 
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KW  - optical tweezers; phage phi 6; RNA packaging; dsRNA virus; RNA BACTERIOPHAGE PHI-6; PLUS-STRAND SYNTHESIS; 3 GENOMIC SEGMENTS; DSRNA BACTERIOPHAGE-PHI-6; POLYMERASE COMPLEX; SYMMETRY MISMATCH; PURIFIED PROTEIN; INTERNAL FORCE; BINDING-SITES; MINUS-STRAND; 1182 Biochemistry, cell and molecular biology; 114 Physical sciences 
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