TY - T1 - Thermocontrolled Reversible Enzyme Complexation-Inactivation-Protection by Poly(N-acryloyl glycinamide) SN - / UR - http://hdl.handle.net/10138/335607 T3 - A1 - Semenyuk, Pavel I.; Kurochkina, Lidia P.; Mäkinen, Lauri; Muronetz, Vladimir I.; Hietala, Sami A2 - PB - Multidisciplinary Digital Publishing Institute Y1 - 2021 LA - AB - A prospective technology for reversible enzyme complexation accompanied with its inactivation and protection followed by reactivation after a fast thermocontrolled release has been demonstrated. A thermoresponsive polymer with upper critical solution temperature, poly(N-acryloyl glycinamide) (PNAGA), which is soluble in water at elevated temperatures but phase separates at low temperatures, has been shown to bind lysozyme, chosen as a model enzyme, at a low temperature (10 °C and lower) b... VO - IS - SP - OP - KW - N1 - PP - ER -