Attenuated Semliki Forest virus for cancer treatment in dogs: safety assessment in two laboratory Beagles

Show full item record



Permalink

http://hdl.handle.net/10138/155706

Citation

BMC Veterinary Research. 2015 Jul 28;11(1):170

Title: Attenuated Semliki Forest virus for cancer treatment in dogs: safety assessment in two laboratory Beagles
Author: Autio, Karoliina P M; Ruotsalainen, Janne J; Anttila, Marjukka O; Niittykoski, Minna; Waris, Matti; Hemminki, Akseli; Vähä-Koskela, Markus J V; Hinkkanen, Ari E
Publisher: BioMed Central
Date: 2015-07-28
URI: http://hdl.handle.net/10138/155706
Abstract: Abstract Background Dogs suffer from spontaneous tumors which may be amenable to therapies developed for human cancer patients, and dogs may serve as large-animal cancer models. A non-pathogenic Semliki Forest virus vector VA7-EGFP previously showed promise in targeting human tumor xenografts in mice, but the oncolytic capacity of the virus in canine cancer cells and the safety of the virus in higher mammals such as dogs, are not known. We therefore assessed the oncolytic potency of VA7-EGFP against canine cancer cells by infectivity and viability assays in two dog solid tumor cell lines. Furthermore we performed a 3-week safety study in two adult Beagles which received a single intravenous injection of ~2 × 105 plaque forming units of parental A7(74) strain. Results VA7-EGFP was able to replicate in and kill both canine cancer cell lines tested. No adverse events were observed in either of the two virus-injected adult Beagles and no infective virus could be recovered from any of the biological samples collected over the course of the study. Neutralizing antibodies to Semliki Forest virus became detectable in the dogs at 5 days post infection and remained elevated until study termination. Conclusions Based on these results, testing of the oncolytic potential of attenuated Semliki Forest virus in canine cancer patients appears feasible.
Rights: Autio et al.


Files in this item

Total number of downloads: Loading...

Files Size Format View
12917_2015_Article_498.pdf 932.2Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record