Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli

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Itkonen , J M , Urtti , A , Bird , L E & Sarkhel , S 2014 , ' Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli ' , BMC Biotechnology , vol. 14 , 92 . https://doi.org/10.1186/s12896-014-0092-x

Title: Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli
Author: Itkonen, Jaakko M.; Urtti, Arto; Bird, Louise E.; Sarkhel, Sanjay
Other contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy



Date: 2014
Language: eng
Number of pages: 11
Belongs to series: BMC Biotechnology
ISSN: 1472-6750
DOI: https://doi.org/10.1186/s12896-014-0092-x
URI: http://hdl.handle.net/10138/162838
Subject: Neurotrophic factors
Human CNTF
Codon optimization
Recombinant soluble expression
E. coli
RECOMBINANT PROTEIN EXPRESSION
HETEROLOGOUS PROTEINS
NEURONOTROPHIC FACTOR
FUSION TECHNOLOGY
GENE-EXPRESSION
FACTOR CNTF
PURIFICATION
SOLUBILITY
SYSTEMS
STRATEGIES
1182 Biochemistry, cell and molecular biology
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