Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)

Show full item record



Permalink

http://hdl.handle.net/10138/163036

Citation

Kovalchuk , A , Raffaello , T , Jaber , E , Keriö , S , Ghimire , R , Lorenz , W W , Dean , J F D , Holopainen , J K & Asiegbu , F O 2015 , ' Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis) ' , BMC Genomics , vol. 16 , 352 . https://doi.org/10.1186/s12864-015-1546-9

Title: Activation of defence pathways in Scots pine bark after feeding by pine weevil (Hylobius abietis)
Author: Kovalchuk, Andriy; Raffaello, Tommaso; Jaber, Emad; Keriö, Susanna; Ghimire, Rajendra; Lorenz, W. Walter; Dean, Jeffrey F. D.; Holopainen, Jarmo K.; Asiegbu, Fred O.
Other contributor: University of Helsinki, Department of Forest Sciences
University of Helsinki, Department of Forest Sciences
University of Helsinki, Department of Forest Sciences
University of Helsinki, Department of Forest Sciences
University of Helsinki, Department of Forest Sciences



Date: 2015-05-06
Language: eng
Number of pages: 15
Belongs to series: BMC Genomics
ISSN: 1471-2164
DOI: https://doi.org/10.1186/s12864-015-1546-9
URI: http://hdl.handle.net/10138/163036
Abstract: Background: During their lifetime, conifer trees are exposed to numerous herbivorous insects. To protect themselves against pests, trees have developed a broad repertoire of protective mechanisms. Many of the plant's defence reactions are activated upon an insect attack, and the underlying regulatory mechanisms are not entirely understood yet, in particular in conifer trees. Here, we present the results of our studies on the transcriptional response and the volatile compounds production of Scots pine (Pinus sylvestris) upon the large pine weevil (Hylobius abietis) feeding. Results: Transcriptional response of Scots pine to the weevil attack was investigated using a novel customised 36.4 K Pinus taeda microarray. The weevil feeding caused large-scale changes in the pine transcriptome. In total, 774 genes were significantly up-regulated more than 4-fold (p = 0.05), whereas 64 genes were significantly down-regulated more than 4-fold. Among the up-regulated genes, we could identify genes involved in signal perception, signalling pathways, transcriptional regulation, plant hormone homeostasis, secondary metabolism and defence responses. The weevil feeding on stem bark of pine significantly increased the total emission of volatile organic compounds from the undamaged stem bark area. The emission levels of monoterpenes and sesquiterpenes were also increased. Interestingly, we could not observe any correlation between the increased production of the terpenoid compounds and expression levels of the terpene synthase-encoding genes. Conclusions: The obtained data provide an important insight into the transcriptional response of conifer trees to insect herbivory and illustrate the massive changes in the host transcriptome upon insect attacks. Moreover, many of the induced pathways are common between conifers and angiosperms. The presented results are the first ones obtained by the use of a microarray platform with an extended coverage of pine transcriptome (36.4 K cDNA elements). The platform will further facilitate the identification of resistance markers with the direct relevance for conifer tree breeding.
Subject: Herbivory
VOC emission
Transcriptomics
Phenylpropanoid pathway
Terpenoid pathway
Protease inhibitors
PR proteins
SPRUCE PICEA SPP.
CONIFER DEFENSE
TRANSCRIPTION FACTORS
GENE-EXPRESSION
SITKA SPRUCE
FUNCTIONAL-CHARACTERIZATION
ARABIDOPSIS-THALIANA
DIRIGENT PROTEINS
STRESS RESPONSES
PISSODES-STROBI
1184 Genetics, developmental biology, physiology
4112 Forestry
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
art_3A10.1186_2Fs12864_015_1546_9.pdf 1005.Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record