A Molecular-Level Account of the Antigenic Hantaviral Surface

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Li , S , Rissanen , I , Zeltina , A , Hepojoki , J , Raghwani , J , Harlos , K , Pybus , O G , Huiskonen , J T & Bowden , T A 2016 , ' A Molecular-Level Account of the Antigenic Hantaviral Surface ' , Cell Reports , vol. 15 , no. 5 , pp. 959-967 . https://doi.org/10.1016/j.celrep.2016.03.082

Title: A Molecular-Level Account of the Antigenic Hantaviral Surface
Author: Li, Sai; Rissanen, Ilona; Zeltina, Antra; Hepojoki, Jussi; Raghwani, Jayna; Harlos, Karl; Pybus, Oliver G.; Huiskonen, Juha T.; Bowden, Thomas A.
Contributor: University of Helsinki, University of Oxford
University of Helsinki, Medicum
University of Helsinki, University of Oxford
Date: 2016-05-03
Language: eng
Number of pages: 9
Belongs to series: Cell Reports
ISSN: 2211-1247
URI: http://hdl.handle.net/10138/163546
Abstract: Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.
Subject: VIRUS ENVELOPE GLYCOPROTEINS
HANTAAN-VIRUS
PUUMALA-VIRUS
MONOCLONAL-ANTIBODIES
CRYO-EM
HEMORRHAGIC-FEVER
PROTEIN
SEQUENCE
NEUTRALIZATION
INFECTIONS
3111 Biomedicine
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