Stomatal Regulation in Transgenic Hybrid Aspen (Populus tremula L. × P. tremuloides Michx) Expressing Arabidopsis SLAC1

Show full item record



Permalink

http://urn.fi/URN:NBN:fi:hulib-201606092261
Title: Stomatal Regulation in Transgenic Hybrid Aspen (Populus tremula L. × P. tremuloides Michx) Expressing Arabidopsis SLAC1
Author: Manjur, A. B. M. Kaiser
Contributor: University of Helsinki, Faculty of Agriculture and Forestry, Department of Agricultural Sciences
Publisher: Helsingfors universitet
Date: 2016
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-201606092261
http://hdl.handle.net/10138/163741
Thesis level: master's thesis
Discipline: Växtproduktionsvetenskap
Plant Production Science
Kasvintuotantotieteet
Abstract: Stomata are microscopic pores; surrounded by a pair of guard cells; they play a crucial role in minimizing the trade-off between conservation of water and photosynthetic efficiency. SLAC1, a stomatal anion channel protein mediates stomatal closure in response to elevated CO2 concentration. Genetic evidence suggested that Populus SLAC1 might have lost its function. In this study hybrid aspen (P. tremula L. × P. tremuloides Michx.) clone 51 was transformed by introducing Arabidopsis thaliana SLAC1 gene regulated by either SLAC1 or GC1 promoter. The aim was to find out guard cell specific promoter and to select transgenic lines that showed rapid stomatal closure in response to elevated CO2 concentration. Histochemical GUS assay suggested that the SLAC1 promoter is more guard cell specific than GC1 promoter. The gas exchange experiment showed an overall decrease in stomatal conductance in transgenic lines with the increasing CO2 concentration compared to wild type plants. However, it was difficult to select the strongest transgenic lines, as all the replicates of independent lines did not show clear response of stomatal closure; in response to elevated CO2 concentration.
Subject: Stomata
SLAC1
Hybrid Aspen
Gas Exchange


Files in this item

Total number of downloads: Loading...

Files Size Format View
Masters_Thesis_A_B_M_Kaiser_Manjur.pdf 2.075Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record