A novel human leiomyoma tissue derived matrix for cell culture studies

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http://hdl.handle.net/10138/164042

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Salo , T , Sutinen , M , Apu , E H , Sundquist , E , Cervigne , N K , de Oliveira , C E , Akram , S U , Ohlmeier , S , Suomi , F , Eklund , L , Juusela , P , Astrom , P , Bitu , C C , Santala , M , Savolainen , K , Korvala , J , Paes Leme , A F & Coletta , R D 2015 , ' A novel human leiomyoma tissue derived matrix for cell culture studies ' , BMC Cancer , vol. 15 , 981 . https://doi.org/10.1186/s12885-015-1944-z

Title: A novel human leiomyoma tissue derived matrix for cell culture studies
Author: Salo, Tuula; Sutinen, Meeri; Apu, Ehsanul Hoque; Sundquist, Elias; Cervigne, Nilva K.; de Oliveira, Carine Ervolino; Akram, Saad Ullah; Ohlmeier, Steffen; Suomi, Fumi; Eklund, Lauri; Juusela, Pirjo; Astrom, Pirjo; Bitu, Carolina Cavalcante; Santala, Markku; Savolainen, Kalle; Korvala, Johanna; Paes Leme, Adriana Franco; Coletta, Ricardo D.
Contributor: University of Helsinki, Clinicum
University of Helsinki, Brendan Battersby / Principal Investigator
Date: 2015-12-16
Language: eng
Number of pages: 16
Belongs to series: BMC Cancer
ISSN: 1471-2407
URI: http://hdl.handle.net/10138/164042
Abstract: Background: The composition of the matrix molecules is important in in vitro cell culture experiments of e.g. human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel (R), are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel (R) is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel. Methods: A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel (R). Myogel was tested and compared to Matrigel (R) in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. Results: We demonstrated that only 34 % of Myogel's molecular content was similar to Matrigel (R). All test results showed that Myogel was comparable with Matrigel (R), and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel (R) in in vitro Transwell (R) invasion and capillary formation assays. Conclusions: In conclusion, we have developed a novel Myogel TME matrix, which is recommended for in vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers.
Subject: Tumor microenvironment matrix
Invasion
Migration
Hanging drop
Colony formation
Spheroid formation
Capillary formation
TUMOR MICROENVIRONMENT
IN-VITRO
EXTRACELLULAR-MATRIX
CANCER-CELLS
MODEL
INVASION
GROWTH
IDENTIFICATION
EXPRESSION
PROTEINS
3122 Cancers
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