A non-targeted metabolite profiling pilot study suggests that tryptophan and lipid metabolisms are linked with ADHD-like behaviours in dogs

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Puurunen , J , Sulkama , S , Tiira , K , Araujo , C , Lehtonen , M , Hanhineva , K & Lohi , H 2016 , ' A non-targeted metabolite profiling pilot study suggests that tryptophan and lipid metabolisms are linked with ADHD-like behaviours in dogs ' , Behavioral and Brain Functions , vol. 12 , 27 . https://doi.org/10.1186/s12993-016-0112-1

Title: A non-targeted metabolite profiling pilot study suggests that tryptophan and lipid metabolisms are linked with ADHD-like behaviours in dogs
Author: Puurunen, Jenni; Sulkama, Sini; Tiira, Katriina; Araujo, Cesar; Lehtonen, Marko; Hanhineva, Kati; Lohi, Hannes
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Veterinary Biosciences
University of Helsinki, Departments of Faculty of Veterinary Medicine
Date: 2016-09-29
Language: eng
Number of pages: 13
Belongs to series: Behavioral and Brain Functions
ISSN: 1744-9081
URI: http://hdl.handle.net/10138/167993
Abstract: Background: Attention deficit hyperactivity disorder (ADHD) is a prevalent and multifactorial neuropsychiatric disorder in the human population worldwide. Complex etiology and clinical heterogeneity have challenged the research, diagnostics and treatment of the disease. Hyperactive and impulsive behaviour has also been observed in dogs, and they could offer a physiologically relevant model for human ADHD. As a part of our ongoing study to understand the molecular etiology of canine anxiety traits, this study was aimed to pilot an approach to identify metabolic biomarkers in canine ADHD-like behaviours for research, diagnostics and treatment purposes. Methods: We collected fresh plasma samples from 22 German Shepherds with varying ADHD-like behaviours. All dogs were on the same controlled diet for 2 weeks prior to sampling. A liquid chromatography combined with mass spectrometry (LC-MS)-based non-targeted metabolite profiling was performed to identify plasma metabolites correlating with the ADHD-like behaviour of the dogs. Results: 649 molecular features correlated with ADHD-like behavioural scores (p(raw) <0.05), and three of them [sn-1 LysoPC(18: 3), PC(18: 3/18: 2) and sn-1 LysoPE(18: 2)] had significant correlations also after FDR correction (pFDR <0.05). Phospholipids were found to negatively correlate with ADHD-like behavioural scores, whereas tryptophan metabolites 3-indolepropionic acid (IPA) and kynurenic acid (KYNA) had negative and positive correlations with ADHD-like behavioural scores, respectively. Conclusions: Our study identified associations between canine ADHD-like behaviours and metabolites that are involved in lipid and tryptophan metabolisms. The identified metabolites share similarity with earlier findings in human and rodent ADHD models. However, a larger replication study is warranted to validate the discoveries prior to further studies to understand the biological role of the identified metabolites in canine ADHD-like behaviours.
Subject: Dog
ADHD
Non-targeted metabolite profiling
Metabolomics
DEFICIT HYPERACTIVITY DISORDER
ATTENTION-DEFICIT/HYPERACTIVITY DISORDER
FATTY-ACID-COMPOSITION
RAT FRONTAL-CORTEX
RED-BLOOD-CELLS
KYNURENINE PATHWAY
MASS-SPECTROMETRY
INDOLE-3-PROPIONIC ACID
ADOLESCENT PATIENTS
MAJOR DEPRESSION
413 Veterinary science
3112 Neurosciences
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