Native and recombinant production of the glucuronoyl esterase from the litter decomposing fungus Stropharia coronilla

Show full item record



Permalink

http://urn.fi/URN:NBN:fi:hulib-201611092976
Title: Native and recombinant production of the glucuronoyl esterase from the litter decomposing fungus Stropharia coronilla
Author: Sun, Mengyi
Contributor: University of Helsinki, Faculty of Agriculture and Forestry, Department of Food and Environmental Sciences
Publisher: Helsingfors universitet
Date: 2016
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-201611092976
http://hdl.handle.net/10138/169771
Thesis level: master's thesis
Discipline: Bioteknik (EYT)
Biotechnology (EYT)
Biotekniikka (EYT)
Abstract: As the sustainable energy is becoming increasingly important, utilization of lignocellulosic biomass for biofuel production is the central part of this area. Fungal enzymes play an important role in lignocellulose degradation. Glucuronoyl esterase (GE) is a less studied fungal enzyme which degrades the ester linkage between lignin alcohol and hemicellulose side chain 4-O-methyl D-glucuronic acids. Genes encoding GE have been identified from various fungal species and they have been expressed in different production systems to be able to study their biochemical properties in detail. The gene encoding GE from the basidiomycete litter-decomposing fungus Stropharia coronilla was cloned and heterologous expressed in Pichia pastoris yeast. The expression and secretion of GE was induced by growing S. coronilla on lignocellulose supplemented cultivations. ScGE activity can be detected after the fifth day cultivation and it peaked on the 14th day. The heterologous expression of ScGE in P. pastoris showed that ScGE was produced as an enzymatically active protein. The commercial K-URONIC kit supplemented with a GE specific substrate benzyl-D-glucuronate was used to determine GE activity.
Subject: Stropharia coronilla
Pichia pastoris
Lignocellulose
Glucuronoyl esterase
Heterologous expression
Enzymatic activity


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show full item record