Postmortem blood concentrations of sartans measured by liquid chromatography-tandem mass spectrometry

Show full item record



Permalink

http://hdl.handle.net/10138/224095

Citation

Ranta , M & Ketola , R 2016 , ' Postmortem blood concentrations of sartans measured by liquid chromatography-tandem mass spectrometry ' , Forensic toxicology , vol. 34 , no. 2 , pp. 235-243 . https://doi.org/10.1007/s11419-016-0308-y

Title: Postmortem blood concentrations of sartans measured by liquid chromatography-tandem mass spectrometry
Author: Ranta, Merja; Ketola, Raimo
Other contributor: University of Helsinki, Medicum
University of Helsinki, Medicum

Date: 2016-07
Language: eng
Number of pages: 9
Belongs to series: Forensic toxicology
ISSN: 1860-8965
DOI: https://doi.org/10.1007/s11419-016-0308-y
URI: http://hdl.handle.net/10138/224095
Abstract: A liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the analysis of seven common sartans (candesartan, eprosartan, losartan, olmesartan, telmisartan, valsartan, and losartan carboxylic acid (EXP3174), the active metabolite of losartan) in postmortem blood samples with liquid-liquid extraction. Detection was accomplished by triple-quadrupole MS in the selected reaction monitoring mode. The validation procedure showed low limits of quantitation of 5 ng/mL for all compounds with good accuracy and precision. The matrix effect was evaluated from the variation of peak areas and concentrations. The method shows some matrix effect but the effect was efficiently compensated by using an internal standard method. The method was applied to the analysis of sartans in postmortem blood samples, and produced 534 positive findings during 2014-2015. Nine deaths were encountered where the concentration of a sartan was very high, indicating intoxication together with other causes of death or multi-drug intoxication. The quantitative results indicate that sartans do not show significant postmortem redistribution; thus concentrations higher than the therapeutic range can be considered as being toxic or fatal.
Subject: Sartans
Angiotensin II receptor antagonists
LC-MS/MS
Postmortem
Blood concentration
II RECEPTOR ANTAGONISTS
SOLID-PHASE EXTRACTION
ANGIOTENSIN-II
HUMAN URINE
HUMAN PLASMA
LOSARTAN
TELMISARTAN
DRUGS
HPLC
PHARMACOKINETICS
319 Forensic science and other medical sciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Postmortem_blood_concentrations.pdf 690.5Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record