Mouse models for human intestinal microbiota research : a critical evaluation

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Hugenholtz , F & de Vos , W M 2018 , ' Mouse models for human intestinal microbiota research : a critical evaluation ' , Cellular and Molecular Life Sciences , vol. 75 , no. 1 , pp. 149-160 . https://doi.org/10.1007/s00018-017-2693-8

Title: Mouse models for human intestinal microbiota research : a critical evaluation
Author: Hugenholtz, Floor; de Vos, Willem M.
Contributor: University of Helsinki, Medicum
Date: 2018-01
Language: eng
Number of pages: 12
Belongs to series: Cellular and Molecular Life Sciences
ISSN: 1420-682X
URI: http://hdl.handle.net/10138/231776
Abstract: Since the early days of the intestinal microbiota research, mouse models have been used frequently to study the interaction of microbes with their host. However, to translate the knowledge gained from mouse studies to a human situation, the major spatio-temporal similarities and differences between intestinal microbiota in mice and humans need to be considered. This is done here with specific attention for the comparative physiology of the intestinal tract, the effect of dietary patterns and differences in genetics. Detailed phylogenetic and metagenomic analysis showed that while many common genera are found in the human and murine intestine, these differ strongly in abundance and in total only 4% of the bacterial genes are found to share considerable identity. Moreover, a large variety of murine strains is available yet most of the microbiota research is performed in wild-type, inbred strains and their transgenic derivatives. It has become increasingly clear that the providers, rearing facilities and the genetic background of these mice have a significant impact on the microbial composition and this is illustrated with recent experimental data. This may affect the reproducibility of mouse microbiota studies and their conclusions. Hence, future studies should take these into account to truly show the effect of diet, genotype or environmental factors on the microbial composition.
Subject: Microbiome
Metagenome
Phylogeny
Murine models
Reproducibility
Diet
SEGMENTED FILAMENTOUS BACTERIA
DISTAL GUT MICROBIOME
DIET-INDUCED OBESITY
GASTROINTESTINAL-TRACT
MUCUS LAYERS
AKKERMANSIA-MUCINIPHILA
COLONIC BIOPSIES
KEY ROLE
MICE
DIVERSITY
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
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