Desmoglein 3-Influence on oral carcinoma cell migration and invasion

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http://hdl.handle.net/10138/243574

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Apu , E H , Akram , S U , Rissanen , J , Wan , H & Salo , T 2018 , ' Desmoglein 3-Influence on oral carcinoma cell migration and invasion ' , Experimental Cell Research , vol. 370 , no. 2 , pp. 353-364 . https://doi.org/10.1016/j.yexcr.2018.06.037

Title: Desmoglein 3-Influence on oral carcinoma cell migration and invasion
Author: Apu, Ehsanul Hoque; Akram, Saad Ullah; Rissanen, Jouni; Wan, Hong; Salo, Tuula
Contributor: University of Helsinki, HUSLAB
Date: 2018-09-15
Language: eng
Number of pages: 12
Belongs to series: Experimental Cell Research
ISSN: 0014-4827
URI: http://hdl.handle.net/10138/243574
Abstract: Desmoglein 3 (Dsg3) is an adhesion receptor in desmosomes, but its role in carcinoma cell migration and invasion is mostly unknown. Our aim was to quantitatively analyse the motion of Dsg3-modified carcinoma cells in 2D settings and in 3D within tumour microenvironment mimicking (TMEM) matrices. We tested mutant constructs of C-terminally truncated Dsg3 (Delta 238 and Delta 560), overexpressed full-length (FL) Dsg3, and empty vector control (Ct) of buccal mucosa squamous cell carcinoma (SqCC/Y1) cells. We captured live cell images and analysed migration velocities and accumulated and Euclidean distances. We compared rodent collagen and Matrigel. with human Myogel TMEM matrices for these parameters in 3D sandwich, in which we also tested the effects of monoclonal antibody AK23, which targets the EC1 domain of Dsg3. In monolayer culture, FL and both truncated constructs migrated faster and had higher accumulated distances than Ct cells. However, in the 3D assays, only the mutants invaded faster relative to Ct cells. Of the mutants, the shorter form (Delta 238) exhibited faster migration and invasion than Delta 560 cells. In the Transwell, all of the cells invaded faster through Myogel than Matrigel coated wells. In 3D sandwich, AK23 antibody inhibited only the invasion of FL cells. We conclude that different experimental 2D and 3D settings can markedly influence the movement of oral carcinoma cells with various Dsg3 modifications.
Subject: Desmoglein 3
3D in vitro model
Co-culture
Myogel
Migration
Invasion
Live cell imaging
Cell tracking
Image analysis
Monoclonal antibody testing
TUMOR MICROENVIRONMENT
EXTRACELLULAR-MATRIX
CANCER PROGRESSION
PROTEIN-KINASE
STROMA
HEAD
INTERFACE
PHENOTYPE
ADHESION
TARGET
3122 Cancers
1182 Biochemistry, cell and molecular biology
313 Dentistry
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