A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium

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Luukkainen , A , Puan , K J , Yusof , N , Lee , B , Tan , K S , Liu , J , Yan , Y , Toppila-Salmi , S , Renkonen , R , Chow , V T , Rotzschke , O & Wang , D Y 2018 , ' A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium ' , Frontiers in Immunology , vol. 9 , 2514 . https://doi.org/10.3389/fimmu.2018.02514

Title: A Co-culture Model of PBMC and Stem Cell Derived Human Nasal Epithelium Reveals Rapid Activation of NK and Innate T Cells Upon Influenza A Virus Infection of the Nasal Epithelium
Author: Luukkainen, Annika; Puan, Kia Joo; Yusof, Nurhashikin; Lee, Bernett; Tan, Kai Sen; Liu, Jing; Yan, Yan; Toppila-Salmi, Sanna; Renkonen, Risto; Chow, Vincent T.; Rotzschke, Olaf; Wang, De Yun
Contributor: University of Helsinki, Medicum
University of Helsinki, Department of Pathology
University of Helsinki, HUSLAB
Date: 2018-11-08
Language: eng
Number of pages: 10
Belongs to series: Frontiers in Immunology
ISSN: 1664-3224
URI: http://hdl.handle.net/10138/265103
Abstract: Background: We established an in vitro co-culture model involving H3N2-infection of human nasal epithelium with peripheral blood mononuclear cells (PBMC) to investigate their cross-talk during early H3N2 infection. Methods: Nasal epithelium was differentiated from human nasal epithelial stem/progenitor cells and cultured wtih fresh human PBMC. PBMC and supernatants were harvested after 24 and 48 h of co-culture with H3N2-infected nasal epithelium. We used flow cytometry and Luminex to characterize PBMC subpopulations, their activation and secretion of cytokine and chemokines. Results: H3N2 infection of the nasal epithelium associated with significant increase in interferons (IFN-alpha, IFN-gamma, IL-29), pro-inflammatory cytokines (TNF-alpha, BDNF, IL-3) and viral-associated chemokines (IP-10, MCP-3, I-TAC, MIG), detectable already after 24 h. This translates into rapid activation of monocytes, NK-cells and innate T-cells (MAIT and gamma delta T cells), evident with CD38+ and/or CD69+ upregulation. Conclusions: This system may contribute to in vitro mechanistic immunological studies bridging systemic models and possibly enable the development of targeted immunomodulatory therapies.
Subject: influenza A virus
peripheral blood mononuclear cells
nasal epithelium
co-culture
innate T cells
IMMUNE-RESPONSES
IN-VITRO
DIFFERENTIAL RESPONSES
RHINOVIRUS
CYTOMEGALOVIRUS
VACCINATION
3111 Biomedicine
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