A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women

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Rekker , K , Altmae , S , Suhorutshenko , M , Peters , M , Martinez-Blanch , J F , Codoner , F M , Vilella , F , Simon , C , Salumets , A & Velthut-Meikas , A 2018 , ' A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women ' , Genes , vol. 9 , no. 12 , 574 . https://doi.org/10.3390/genes9120574

Title: A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women
Author: Rekker, Kadri; Altmae, Signe; Suhorutshenko, Marina; Peters, Maire; Martinez-Blanch, Juan F.; Codoner, Francisco M.; Vilella, Felipe; Simon, Carlos; Salumets, Andres; Velthut-Meikas, Agne
Contributor: University of Helsinki, Clinicum
Date: 2018-12
Language: eng
Number of pages: 17
Belongs to series: Genes
ISSN: 2073-4425
URI: http://hdl.handle.net/10138/294731
Abstract: The endometrium undergoes extensive changes to prepare for embryo implantation and microRNAs (miRNAs) have been described as playing a significant role in the regulation of endometrial receptivity. However, there is no consensus about the miRNAs involved in mid-secretory endometrial functions. We analysed the complete endometrial miRNome from early secretory (pre-receptive) and mid-secretory (receptive) phases from fertile women and from patients with recurrent implantation failure (RIF) to reveal differentially expressed (DE) miRNAs in the mid-secretory endometrium. Furthermore, we investigated whether the overall changes during early to mid-secretory phase transition and with RIF condition could be reflected in blood miRNA profiles. In total, 116 endometrial and 114 matched blood samples collected from two different population cohorts were subjected to small RNA sequencing. Among fertile women, 91 DE miRNAs were identified in the mid-secretory vs. early secretory endometrium, while no differences were found in the corresponding blood samples. The comparison of mid-secretory phase samples between fertile and infertile women revealed 21 DE miRNAs from the endometrium and one from blood samples. Among discovered novel miRNAs, chr2_4401 was validated and showed up-regulation in the mid-secretory endometrium. Besides novel findings, we confirmed the involvement of miR-30 and miR-200 family members in mid-secretory endometrial functions.
Subject: endometrial receptivity
infertility
microRNA
recurrent implantation failure
small RNA-seq
DIFFERENTIAL EXPRESSION ANALYSIS
RECURRENT IMPLANTATION FAILURE
EMBRYO IMPLANTATION
MICRORNA
TIME
ACTIVATION
SIGNATURE
RESOURCE
PACKAGE
MIR-30D
3123 Gynaecology and paediatrics
3111 Biomedicine
1184 Genetics, developmental biology, physiology
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