Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Show full item record



Permalink

http://hdl.handle.net/10138/299000

Citation

Mgbeahuruike , E E , Fyhrquist , P , Vuorela , H , Julkunen-Tiitto , R & Holm , Y 2018 , ' Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects ' , Antibiotics , vol. 7 , no. 4 , 98 . https://doi.org/10.3390/antibiotics7040098

Title: Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects
Author: Mgbeahuruike, Eunice Ego; Fyhrquist, Pia; Vuorela, Heikki; Julkunen-Tiitto, Riitta; Holm, Yvonne
Contributor: University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences
Date: 2018-12
Language: eng
Number of pages: 18
Belongs to series: Antibiotics
ISSN: 2079-6382
URI: http://hdl.handle.net/10138/299000
Abstract: Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UV absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 mu g/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 mu g/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 mu g/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39-1250 mu g/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.
Subject: Piperamides
antibacterial
Piper guineense
piperine
piperlongumine
MEDICINAL-PLANTS
ANTIMICROBIAL ACTIVITY
BLACK PEPPER
EFFLUX PUMP
IDENTIFICATION
HPLC
L.
317 Pharmacy
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
antibiotics_07_00098.pdf 758.4Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record