Lactate inhibits germ cell apoptosis in the human testis

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Erkkilä , K , Aito , H , Aalto , K , Pentikäinen , V & Dunkel , L 2002 , ' Lactate inhibits germ cell apoptosis in the human testis ' , Molecular Human Reproduction , vol. 8 , no. 2 , pp. 109-117 . https://doi.org/10.1093/molehr/8.2.109

Title: Lactate inhibits germ cell apoptosis in the human testis
Author: Erkkilä, K.; Aito, H.; Aalto, K.; Pentikäinen, V.; Dunkel, L.
Contributor organization: Children's Hospital
Clinicum
Lastentautien yksikkö
Date: 2002-02
Language: eng
Number of pages: 9
Belongs to series: Molecular Human Reproduction
ISSN: 1360-9947
DOI: https://doi.org/10.1093/molehr/8.2.109
URI: http://hdl.handle.net/10138/299717
Abstract: Dysregulation of male germ cell apoptosis has been associated with the pathogenesis of male infertility. Therefore, factors involved in the regulation of germ cell death are being actively investigated. Here, we studied the effects of lactate on human male germ cell death, using as a model a testis tissue culture in which physiological contacts are maintained between the germ cells and the supportive somatic Sertoli cells. Apoptosis of spermatocytes, spermatids and a few spermatogonia was induced by culturing segments of seminiferous tubules under serum-free conditions. This germ cell death was inhibited effectively and dose-dependently by lactate, indicating that it plays a crucial role in controlling cell death cascades of male germ cells. Interestingly, the anti-apoptotic role of lactate was not associated with changes in testicular adenine nucleotide (ATP, ADP and AMP) levels. In the seminiferous tubules, the final site of the death-suppressing action of lactate appeared to be downstream along the cell death pathway activated by the Fas receptor of the germ cells. In conclusion, testicular cell death was effectively regulated by lactate, which may be regarded as a potential compound for optimizing in-vitro methods involving male germ cells for assisted reproduction.
Subject: apoptosis
germ cell
human testis
lactate
spermatogenesis
HUMAN CHORIONIC-GONADOTROPIN
IN-VITRO
SERTOLI CELLS
PERMEABILITY TRANSITION
RAT TESTIS
STEM-CELLS
SPERMATOGENESIS
DEATH
SURVIVAL
METABOLISM
1184 Genetics, developmental biology, physiology
3123 Gynaecology and paediatrics
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion


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