Spreading of Cell Aggregates on Zwitterion-Modified Chitosan Films

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Qi , B , Feng , H , Qiu , X , Beaune , G , Guo , X , Brochard-Wyart , F & Winnik , F M 2019 , ' Spreading of Cell Aggregates on Zwitterion-Modified Chitosan Films ' , Langmuir , vol. 35 , no. 5 , pp. 1902-1908 . https://doi.org/10.1021/acs.langmuir.8b02461

Title: Spreading of Cell Aggregates on Zwitterion-Modified Chitosan Films
Author: Qi, Baowen; Feng, Haike; Qiu, Xingping; Beaune, Gregory; Guo, Xiaoqiang; Brochard-Wyart, Francoise; Winnik, Francoise M.
Contributor organization: Polymers
Department of Chemistry
Date: 2019-02-05
Language: eng
Number of pages: 7
Belongs to series: Langmuir
ISSN: 0743-7463
DOI: https://doi.org/10.1021/acs.langmuir.8b02461
URI: http://hdl.handle.net/10138/300065
Abstract: The sulfobetaine (SB) moiety, which comprises a quaternary ammonium group linked to a negatively charged sulfonate ester, is known to impart nonfouling properties to interfaces coated with polysulfobetaines or grafted with SB-polymeric brushes. Increasingly, evidence emerges that the SB group is, overall, a better antifouling group than the phosphorylcholine (PC) moiety extensively used in the past. We report here the synthesis of a series of SB-modified chitosans (CH-SB) carrying between 20 and 40 mol % SB per monosaccharide unit. Chitosan (CH) itself is a naturally derived copolymer of glucosamine and N-acetyl-glucosamine linked with a beta-1,4 bond. Analysis by quartz crystal microbalance with dissipation (QCM-D) indicates that CH-SB films (thickness similar to 20 nm) resist adsorption of bovine serum albumin (BSA) with increasing efficiency as the SB content of the polymer augments (surface coverage similar to 15 mu g cm(-2) for films of CH with 40 mol % SB). The cell adhesivity of CH-SB films coated on glass was assessed by determining the spreading dynamics of CT26 cell aggregates. When placed on chitosan films, known to be cell-adhesive, the CT26 cell aggregates spread by forming a cell monolayer around them. The spreading of CT26 cell aggregates on zwitterion-modified chitosans films is thwarted remarkably. In the cases of CH-SB30 and CH-SB40 films, only a few isolated cells escape from the aggregates. The extent of aggregate spreading, quantified based on the theory of liquid wetting, provides a simple in vitro assay of the nonfouling properties of substrates toward specific cell lines. This assay can be adopted to test and compare the fouling characteristics of substrates very different from the chemical viewpoint.
116 Chemical sciences
114 Physical sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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