Molecular determinants of ER-Golgi contacts identified through a new FRET-FLIM system

Show simple item record Venditti, Rossella Rega, Laura Rita Masone, Maria Chiara Santoro, Michele Polishchuk, Elena Sarnataro, Daniela Paladino, Simona D'Auria, Sabato Varriale, Antonio Olkkonen, Vesa M. Di Tullio, Giuseppe Polishchuk, Roman De Matteis, Maria Antonietta 2019-09-29T21:42:13Z 2021-12-17T22:45:30Z 2019-03
dc.identifier.citation Venditti , R , Rega , L R , Masone , M C , Santoro , M , Polishchuk , E , Sarnataro , D , Paladino , S , D'Auria , S , Varriale , A , Olkkonen , V M , Di Tullio , G , Polishchuk , R & De Matteis , M A 2019 , ' Molecular determinants of ER-Golgi contacts identified through a new FRET-FLIM system ' , Journal of Cell Biology , vol. 218 , no. 3 , pp. 1055-1065 .
dc.identifier.other PURE: 123533208
dc.identifier.other PURE UUID: dcea7037-ea92-4a13-81b8-5b3d9ab0ffab
dc.identifier.other WOS: 000460161200028
dc.identifier.other Scopus: 85062418018
dc.description.abstract ER-TGN contact sites (ERTGoCS) have been visualized by electron microscopy, but their location in the crowded perinuclear area has hampered their analysis via optical microscopy as well as their mechanistic study. To overcome these limits we developed a FRET-based approach and screened several candidates to search for molecular determinants of the ERTGoCS. These included the ER membrane proteins VAPA and VAPB and lipid transfer proteins possessing dual (ER and TGN) targeting motifs that have been hypothesized to contribute to the maintenance of ERTGoCS, such as the ceramide transfer protein CERT and several members of the oxysterol binding proteins. We found that VAP proteins, OSBP1, ORP9, and ORP10 are required, with OSBP1 playing a redundant role with ORP9, which does not involve its lipid transfer activity, and ORP10 being required due to its ability to transfer phosphatidylserine to the TGN. Our results indicate that both structural tethers and a proper lipid composition are needed for ERTGoCS integrity. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Journal of Cell Biology
dc.rights cc_by_nc_sa
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject BINDING
dc.subject VAP
dc.subject TRAFFICKING
dc.subject TRANSPORT
dc.subject PROTEINS
dc.subject INTERACTOME
dc.subject 3111 Biomedicine
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Molecular determinants of ER-Golgi contacts identified through a new FRET-FLIM system en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Faculty of Medicine
dc.contributor.organization Department of Anatomy
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0021-9525
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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