A comparative map of macroautophagy and mitophagy in the vertebrate eye

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http://hdl.handle.net/10138/306378

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McWilliams , T G , Prescott , A R , Villarejo-Zori , B , Ball , G , Boya , P & Ganleya , I G 2019 , ' A comparative map of macroautophagy and mitophagy in the vertebrate eye ' , Autophagy , vol. 15 , no. 7 , pp. 1296-1308 . https://doi.org/10.1080/15548627.2019.1580509

Title: A comparative map of macroautophagy and mitophagy in the vertebrate eye
Author: McWilliams, Thomas G.; Prescott, Alan R.; Villarejo-Zori, Beatriz; Ball, Graeme; Boya, Patricia; Ganleya, Ian G.
Contributor: University of Helsinki, Department of Anatomy
Date: 2019-07-03
Language: eng
Number of pages: 13
Belongs to series: Autophagy
ISSN: 1554-8627
URI: http://hdl.handle.net/10138/306378
Abstract: Photoreception is pivotal to our experience and perception of the natural world; hence the eye is of prime importance for most vertebrate animals to sense light. Central to visual health is mitochondrial homeostasis, and the selective autophagic turnover of mitochondria (mitophagy) is predicted to play a key role here. Despite studies that link aberrant mitophagy to ocular dysfunction, little is known about the prevalence of basal mitophagy, or its relationship to general autophagy, in the visual system. In this study, we utilize the mito-QC mouse and a closely related general macroautophagy reporter model to profile basal mitophagy and macroautophagy in the adult and developing eye. We report that ocular macroautophagy is widespread, but surprisingly mitophagy does not always follow the same pattern of occurrence. We observe low levels of mitophagy in the lens and ciliary body, in stark contrast to the high levels of general MAP1LC3-dependent macroautophagy in these regions. We uncover a striking reversal of this process in the adult retina, where mitophagy accounts for a larger degree of the macroautophagy taking place, specifically in the photoreceptor neurons of the outer nuclear layer. We also show the developmental regulation of autophagy in a variety of ocular tissues. In particular, mitophagy in the adult mouse retina is reversed in localization during the latter stages of development. Our work thus defines the landscape of mitochondrial homeostasis in the mammalian eye, and in doing so highlights the selective nature of autophagy in vivo and the specificity of the reporters used.
Subject: Autophagy
ciliary body
cornea
eye
hyaloid
lens
mitochondria
mito-QC
mitophagy
retina
CELL-DEATH
MITOCHONDRIAL DYSFUNCTION
ENERGY-METABOLISM
OXIDATIVE STRESS
VISUAL CELLS
MOUSE MODEL
AUTOPHAGY
LENS
DEGENERATION
ORGANIZATION
1184 Genetics, developmental biology, physiology
1182 Biochemistry, cell and molecular biology
3111 Biomedicine
3112 Neurosciences
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