An evaluation of serological methods to diagnose tick-borne encephalitis from serum and cerebrospinal fluid

Show full item record



Permalink

http://hdl.handle.net/10138/306902

Citation

Reusken , C , Boonstra , M , Rugebregt , S , Scherbeijn , S , Chandler , F , Avsic-Zupanc , T , Vapalahti , O , Koopmans , M & GeurtsvanKessel , C H 2019 , ' An evaluation of serological methods to diagnose tick-borne encephalitis from serum and cerebrospinal fluid ' , Journal of Clinical Virology , vol. 120 , pp. 78-83 . https://doi.org/10.1016/j.jcv.2019.09.009

Title: An evaluation of serological methods to diagnose tick-borne encephalitis from serum and cerebrospinal fluid
Author: Reusken, Chantal; Boonstra, Marrit; Rugebregt, Sharona; Scherbeijn, Sandra; Chandler, Felicity; Avsic-Zupanc, Tatjana; Vapalahti, Olli; Koopmans, Marion; GeurtsvanKessel, Corine H.
Contributor: University of Helsinki, Helsinki One Health (HOH)
Date: 2019-11
Language: eng
Number of pages: 6
Belongs to series: Journal of Clinical Virology
ISSN: 1386-6532
URI: http://hdl.handle.net/10138/306902
Abstract: Background: Tick-borne encephalitis (TBE) is an infectious disease endemic to large parts of Europe and Asia. Diagnosing TBE often relies on the detection of TBEV-specific antibodies in serum and cerebrospinal fluid (CSF) as viral genome is mostly not detectable once neurological symptoms occur. Objectives: We evaluated the performance of TBEV IgM and IgG ELISAs in both serum and CSF of confirmed TBEV patients and discuss the role of (CSF) serology in TBEV diagnostics. Study design: For the assay evaluation we collected specimen from confirmed TBEV patients. Assay specificity was assessed using sera from patients with a related flavivirus infection or other acute infection. A selected ELISA assay was used to analyze TBEV-specific antibodies in CSF and to evaluate the use in confirming TBE diagnosis. Results: In this study the overall sensitivity of the IgM TBEV ELISAs was acceptable (94-100 %). Four out of five IgM ELISA's demonstrated an excellent overall specificity from 94-100% whereas a low overall specificity was observed for the IgG TBEV ELISAs (30-71%). Intrathecal antibody production against TBEV was demonstrated in a subset of TBE patients. Conclusions: In four out of five ELISAs, IgM testing in serum and CSF of TBE patients is specific and confirmative. The lack of IgG specificity in all ELISAs emphasizes the need of confirmatory testing by virus neutralisation, depending on the patient's background and the geographic location of exposure to TBEV. A CSF-serum IgG antibody index can support the diagnosis specifically in chronic disease or once IgM has disappeared.
Subject: Tick-borne encephalitis virus
Serology
ELISA
Immunoassay
Virus neutralisation
Cerebrospinal fluid
Diagnostics
Antibody index
ENZYME-LINKED IMMUNOSORBENT
IGG-ELISA KITS
CROSS-REACTIONS
TBE VIRUS
ANTIBODIES
EPIDEMIOLOGY
EASTERN
FOCUS
3111 Biomedicine
1183 Plant biology, microbiology, virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S1386653219302148_main.pdf 1.074Mb PDF View/Open
1_s2.0_S1386653219302148_main.pdf 1.939Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record