Effect of meningeal lymphatic vessel depletion on T cell-mediated adaptive immune response in traumatic brain injury

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http://urn.fi/URN:NBN:fi:hulib-201912034046
Title: Effect of meningeal lymphatic vessel depletion on T cell-mediated adaptive immune response in traumatic brain injury
Author: Wong, Carlton
Contributor: University of Helsinki, Faculty of Biological and Environmental Sciences
Publisher: Helsingin yliopisto
Date: 2019
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-201912034046
http://hdl.handle.net/10138/308220
Thesis level: master's thesis
Degree program: Neurotieteiden maisteriohjelma
Master's Programme in Neuroscience
Magisterprogrammet i neurovetenskap
Specialisation: Neurotiede
Neuroscience
Neurovetenskap
Abstract: Meningeal lymphatics vessels (mLVs), the recently characterized lymphatics in the central nervous system (CNS), provide a link between the adaptive immune system and the CNS. mLVs could be important for the activation of T cell-mediated adaptive immune response, by draining antigens from the brain to the deep cervical lymph nodes, where they are presented to T cells. In traumatic brain injury (TBI), we hypothesized that the activation of self-reactive T cells (i.e., T cells able to recognize self, brain-derived antigens and promote an immune reaction), possibly underlies the pathogenesis of the disease. In order to test this hypothesis and to decipher the specific role of mLVs in the modulation of T cell-mediated neuro-immune response after TBI, we ablated the existing mLVs in adult male C57BL/6OlaJ mice (with the use of the AAV-mVEGFR3 1-4 Ig vector), induced TBI with controlled cortical impact, and examined the motor function of the mice and the activation of different T cell populations in the brain, as well as in the secondary lymphoid (spleen and lymph nodes – LNs) and non-lymphoid organs (meninges). Our data showed that the T cell-mediated adaptive neuro-immune response in TBI was unaffected by the depletion of mLVs. Our results, however, are preliminary, due to the limited sample size used in this study, which reduces the statistical power and restricts our ability to conclude for the effect of mLV depletion on TBI recovery.
Subject: Central nervous system immunity
Neuroimmune response
Meningeal lymphatic vessels
T cells
Adaptive immune response
Traumatic brain injury


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