Field-flow fractionation of cationic cellulose derivatives

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dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor.author Tenkanen, Maija
dc.contributor.author Pitkänen, Leena
dc.date.accessioned 2020-01-09T13:49:01Z
dc.date.available 2020-01-09T13:49:01Z
dc.date.issued 2019-12
dc.identifier.citation Tenkanen , M & Pitkänen , L 2019 , ' Field-flow fractionation of cationic cellulose derivatives ' , Chromatographia , vol. 82 , no. 12 , pp. 1827-1832 . https://doi.org/10.1007/s10337-019-03800-2 en
dc.identifier.issn 0009-5893
dc.identifier.other PURE: 126418756
dc.identifier.other PURE UUID: fccfe512-ef5b-479a-8c2a-285cfe8f5c3b
dc.identifier.other WOS: 000493609400008
dc.identifier.other ORCID: /0000-0003-2883-2717/work/67132759
dc.identifier.uri http://hdl.handle.net/10138/309185
dc.description.abstract The asymmetric flow field-flow fractionation (AF4) method was developed for cationic cellulose derivatives. AF4 is the method of choice especially for high-molar mass samples, which are challenging to characterize with conventional chromatographic techniques such as size-exclusion chromatography (SEC). The cationic charge of macromolecules also complicates the size-based separations where no interaction between the analytes and the column stationary phase (SEC) or membrane (AF4) should occur. However, many column matrices and membranes carry negative charge and thus preventing interactions between cationic analytes and negatively charged separation support should be taken into consideration when doing method development. In this study, two eluent compositions, neutral and acidic, were tested for AF4 separation of cationic hydroxyethyl celluloses with varying charge densities. The eluent composition with a pH below the isoelectric point of regenerated cellulose membrane, which was used in this AF4 study, enabled the size-based separation with close to 100% analysis recovery. Macromolecular parameters (molar mass and radius of gyration) and conformation were investigated by coupling a multi-angle light scattering detector and differential refractometer to the AF4 system. en
dc.format.extent 6
dc.language.iso eng
dc.relation.ispartof Chromatographia
dc.rights en
dc.subject 416 Food Science en
dc.subject 116 Chemical sciences en
dc.subject Field-flow fractionation en
dc.subject Separation en
dc.subject Cationic polysaccharides en
dc.subject Charge density en
dc.subject POLYMERS en
dc.title Field-flow fractionation of cationic cellulose derivatives en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1007/s10337-019-03800-2
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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