Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry

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http://hdl.handle.net/10138/312560

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Nonnemann , B , Lyhs , U , Svennesen , L , Kristensen , K A , Klaas , I C & Pedersen , K 2019 , ' Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry ' , Journal of Dairy Science , vol. 102 , no. 3 , pp. 2515-2524 . https://doi.org/10.3168/jds.2018-15424

Title: Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry
Author: Nonnemann, Bettina; Lyhs, Ulrike; Svennesen, Line; Kristensen, Katja Ann; Klaas, Ilka C.; Pedersen, Karl
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
Date: 2019-03
Language: eng
Number of pages: 10
Belongs to series: Journal of Dairy Science
ISSN: 0022-0302
URI: http://hdl.handle.net/10138/312560
Abstract: Matrix-assisted laser desorption/ionization timeof-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method to identify the most common pathogenic bacteria in humans and animals. The goals of this study were to amend a commercial database with additional species, evaluate the amended database for identification of bacterial genera and species causing bovine mastitis, and describe the plethora of species involved. In total, 500 udder pathogenic isolates were subjected to MALDI-TOF MS using bacterial or fungal colony material; 93.5% could be identified to the species level, and 6.5% were identified only to the genus level. Isolates identified to the genus level required further identification to the species level by conventional methods or 16S rDNA sequencing. Mass spectra from verified species were used to expand the MALDI-TOF MS database to improve future identification ability. A total of 24 genera and 61 species were identified in this study. Identified isolates were mainly staphylococci, streptococci, Enterobacteriaceae, and coryneforme bacteria. In conclusion, MALDI-TOF MS is a powerful, rapid, and reliable technique to identify the most common microorganisms causing bovine mastitis, and the database can be continuously expanded and improved with additional species.
Subject: bovine mastitis
matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry
rapid identification
DESORPTION IONIZATION-TIME
COAGULASE-NEGATIVE STAPHYLOCOCCI
SMALL-COLONY VARIANTS
ROUTINE IDENTIFICATION
SUBCLINICAL MASTITIS
ENTEROCOCCUS-FAECIUM
CLINICAL MASTITIS
DAIRY HERDS
MILK
INFECTIONS
3111 Biomedicine
412 Animal science, dairy science
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