A Missense Variant Affecting the C-Terminal Tail of UNC93B1 in Dogs with Exfoliative Cutaneous Lupus Erythematosus (ECLE)

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Leeb , T , Leuthard , F , Jagannathan , V , Kiener , S , Letko , A , Roosje , P , Welle , M M , Gailbreath , K L , Cannon , A , Linek , M , Banovic , F , Olivry , T , White , S D , Batcher , K , Bannasch , D , Minor , K M , Mickelson , J R , Hytönen , M K , Lohi , H , Mauldin , E A & Casal , M L 2020 , ' A Missense Variant Affecting the C-Terminal Tail of UNC93B1 in Dogs with Exfoliative Cutaneous Lupus Erythematosus (ECLE) ' , Genes , vol. 11 , no. 2 , 159 . https://doi.org/10.3390/genes11020159

Title: A Missense Variant Affecting the C-Terminal Tail of UNC93B1 in Dogs with Exfoliative Cutaneous Lupus Erythematosus (ECLE)
Author: Leeb, Tosso; Leuthard, Fabienne; Jagannathan, Vidhya; Kiener, Sarah; Letko, Anna; Roosje, Petra; Welle, Monika M.; Gailbreath, Katherine L.; Cannon, Andrea; Linek, Monika; Banovic, Frane; Olivry, Thierry; White, Stephen D.; Batcher, Kevin; Bannasch, Danika; Minor, Katie M.; Mickelson, James R.; Hytönen, Marjo K.; Lohi, Hannes; Mauldin, Elizabeth A.; Casal, Margret L.
Contributor: University of Helsinki, Department of Medical and Clinical Genetics
University of Helsinki, Helsinki One Health (HOH)
Date: 2020-02
Language: eng
Number of pages: 10
Belongs to series: Genes
ISSN: 2073-4425
URI: http://hdl.handle.net/10138/313951
Abstract: Cutaneous lupus erythematosus (CLE) in humans encompasses multiple subtypes that exhibit a wide array of skin lesions and, in some cases, are associated with the development of systemic lupus erythematosus (SLE). We investigated dogs with exfoliative cutaneous lupus erythematosus (ECLE), a dog-specific form of chronic CLE that is inherited as a monogenic autosomal recessive trait. A genome-wide association study (GWAS) with 14 cases and 29 controls confirmed a previously published result that the causative variant maps to chromosome 18. Autozygosity mapping refined the ECLE locus to a 493 kb critical interval. Filtering of whole genome sequence data from two cases against 654 controls revealed a single private protein-changing variant in this critical interval, UNC93B1:c.1438C>A or p.Pro480Thr. The homozygous mutant genotype was exclusively observed in 23 ECLE affected German Shorthaired Pointers and an ECLE affected Vizsla, but absent from 845 controls. UNC93B1 is a transmembrane protein located in the endoplasmic reticulum and endolysosomes, which is required for correct trafficking of several Toll-like receptors (TLRs). The p.Pro480Thr variant is predicted to affect the C-terminal tail of the UNC93B1 that has recently been shown to restrict TLR7 mediated autoimmunity via an interaction with syndecan binding protein (SDCBP). The functional knowledge on UNC93B1 strongly suggests that p.Pro480Thr is causing ECLE in dogs. These dogs therefore represent an interesting spontaneous model for human lupus erythematosus. Our results warrant further investigations of whether genetic variants affecting the C-terminus of UNC93B1 might be involved in specific subsets of CLE or SLE cases in humans and other species.
Subject: Canis familiaris
dermatology
immunology
animal model
skin
TLR7
toll-like receptor
syndecan binding protein
syntenin-1
systemic lupus erythematosus
SLE
CLE
GENOME
POPULATION
CLASSIFICATION
AUTOIMMUNITY
ASSOCIATION
RECEPTOR-7
MUTATION
413 Veterinary science
1184 Genetics, developmental biology, physiology
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