Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017

Show full item record



Albinsson , B , Jääskeläinen , A E , Värv , K , Jelovšek , M , GeurtsvanKessel , C , Vene , S , Järhult , J D , Reusken , C , Golovljova , I , Avšič-Županc , T , Vapalahti , O & Lundkvist , Å 2020 , ' Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017 ' , Eurosurveillance , vol. 25 , no. 12 , 1900427 , pp. 27-36 .

Title: Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017
Author: Albinsson, Bo; Jääskeläinen, Anu E.; Värv, Kairi; Jelovšek, Mateja; GeurtsvanKessel, Corine; Vene, Sirkka; Järhult, Josef D.; Reusken, Chantal; Golovljova, Irina; Avšič-Županc, Tatjana; Vapalahti, Olli; Lundkvist, Åke
Contributor organization: HUSLAB
Viral Zoonosis Research Unit
Department of Virology
Helsinki One Health (HOH)
Veterinary Microbiology and Epidemiology
Veterinary Biosciences
Date: 2020-03-26
Language: eng
Number of pages: 10
Belongs to series: Eurosurveillance
ISSN: 1025-496X
Abstract: Tick-borne encephalitis (TBE) is a potentially severe neurological disease caused by TBE virus (TBEV). In Europe and Asia, TBEV infection has become a growing public health concern and requires fast and specific detection. Aim: In this observational study, we evaluated a rapid TBE IgM test, ReaScan TBE, for usage in a clinical laboratory setting. Methods: Patient sera found negative or positive for TBEV by serological and/or molecular methods in diagnostic laboratories of five European countries endemic for TBEV (Estonia, Finland, Slovenia, the Netherlands and Sweden) were used to assess the sensitivity and specificity of the test. The patients' diagnoses were based on other commercial or quality assured in-house assays, i.e. each laboratory's conventional routine methods. For specificity analysis, serum samples from patients with infections known to cause problems in serology were employed. These samples tested positive for e.g. Epstein-Barr virus, cytomegalovirus and Anaplasma phagocytophilum, or for flaviviruses other than TBEV, i.e. dengue, Japanese encephalitis, West Nile and Zika viruses. Samples from individuals vaccinated against flaviviruses other than TBEV were also included. Altogether, 172 serum samples from patients with acute TBE and 306 TBE IgM negative samples were analysed. Results: Compared with each laboratory's conventional methods, the tested assay had similar sensitivity and specificity (99.4% and 97.7%, respectively). Samples containing potentially interfering antibodies did not cause specificity problems. Conclusion: Regarding diagnosis of acute TBEV infections, ReaScan TBE offers rapid and convenient complementary IgM detection. If used as a stand-alone, it can provide preliminary results in a laboratory or point of care setting.
Subject: 3111 Biomedicine
11832 Microbiology and virology
3142 Public health care science, environmental and occupational health
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
eurosurv_25_12_6.pdf 234.2Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record