Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells

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http://hdl.handle.net/10138/314199

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Wagner , M , Yoshihara , M , Douagi , I , Damdimopoulos , A , Panula , S , Petropoulos , S , Lu , H , Pettersson , K , Palm , K , Katayama , S , Hovatta , O , Kere , J , Lanner , F & Damdimopoulou , P 2020 , ' Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells ' , Nature Communications , vol. 11 , no. 1 , 1147 . https://doi.org/10.1038/s41467-020-14936-3

Title: Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
Author: Wagner, Magdalena; Yoshihara, Masahito; Douagi, Iyadh; Damdimopoulos, Anastasios; Panula, Sarita; Petropoulos, Sophie; Lu, Haojiang; Pettersson, Karin; Palm, Kerstin; Katayama, Shintaro; Hovatta, Outi; Kere, Juha; Lanner, Fredrik; Damdimopoulou, Pauliina
Contributor: University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
Date: 2020-03-02
Language: eng
Number of pages: 15
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/314199
Abstract: The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve. The outer lining or cortex of the human ovary determines fertility and has been postulated to contain oogonial stem cells. Here, the authors generate a single-cell map of the adult human ovarian cortex and show that DDX4 labels perivascular cells but no oogonial stem cells.
Subject: ACTIVE GERM-CELLS
TISSUE CRYOPRESERVATION
ADULT HUMAN
LOCALIZATION
FOLLICLES
GENE
VASA
PRESERVATION
TRANSITION
INJECTION
3111 Biomedicine
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