Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation

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dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Helsinki Institute of Life Science HiLIFE en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor.author Binder, Martin
dc.contributor.author Biggs, Leah C.
dc.contributor.author Kronenberg, Mark S.
dc.contributor.author Schneider, Pascal
dc.contributor.author Thesleff, Irma
dc.contributor.author Balic, Anamaria
dc.date.accessioned 2020-06-24T13:24:01Z
dc.date.available 2020-06-24T13:24:01Z
dc.date.issued 2020-03-18
dc.identifier.citation Binder , M , Biggs , L C , Kronenberg , M S , Schneider , P , Thesleff , I & Balic , A 2020 , ' Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation ' , Scientific Reports , vol. 10 , no. 1 , 4963 . https://doi.org/10.1038/s41598-020-60708-w en
dc.identifier.issn 2045-2322
dc.identifier.other PURE: 135706552
dc.identifier.other PURE UUID: 37487a9f-10fb-472f-8424-988a8fd209d2
dc.identifier.other RIS: urn:FA8ADCCEED4DBEDF88ABC3D967843DDF
dc.identifier.other RIS: Binder2020
dc.identifier.other ORCID: /0000-0001-6796-1197/work/76332662
dc.identifier.other ORCID: /0000-0002-4990-8664/work/76332903
dc.identifier.other WOS: 000563454700012
dc.identifier.uri http://hdl.handle.net/10138/316911
dc.description.abstract Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights en
dc.subject 1184 Genetics, developmental biology, physiology en
dc.title Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1038/s41598-020-60708-w
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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