A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context

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Radhakrishnan , D , Shanmukhan , A P , Kareem , A , Aiyaz , M , Varapparambathu , V , Toms , A , Kerstens , M , Valsakumar , D , Landge , A N , Shaji , A , Mathew , M K , Sawchuk , M G , Scarpella , E , Krizek , B A , Efroni , I , Mähönen , A P , Willemsen , V , Scheres , B & Prasad , K 2020 , ' A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context ' , Development , vol. 147 , no. 6 , 185710 . https://doi.org/10.1242/dev.185710

Title: A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context
Author: Radhakrishnan, Dhanya; Shanmukhan, Anju Pallipurath; Kareem, Abdul; Aiyaz, Mohammed; Varapparambathu, Vijina; Toms, Ashna; Kerstens, Merijn; Valsakumar, Devisree; Landge, Amit N.; Shaji, Anil; Mathew, Mathew K.; Sawchuk, Megan G.; Scarpella, Enrico; Krizek, Beth A.; Efroni, Idan; Mähönen, Ari Pekka; Willemsen, Viola; Scheres, Ben; Prasad, Kalika
Contributor: University of Helsinki, Helsinki Institute of Life Science HiLIFE
Date: 2020-03
Number of pages: 13
Belongs to series: Development
ISSN: 0950-1991
URI: http://hdl.handle.net/10138/317516
Abstract: Aerial organs of plants, being highly prone to local injuries, require tissue restoration to ensure their survival. However, knowledge of the underlying mechanism is sparse. In this study, we mimicked natural injuries in growing leaves and stems to study the reunion between mechanically disconnected tissues. We show that PLETHORA (PLT) and AINTEGUMENTA (ANT) genes, which encode stem cell-promoting factors, are activated and contribute to vascular regeneration in response to these injuries. PLT proteins bind to and activate the CUC2 promoter. PLT proteins and CUC2 regulate the transcription of the local auxin biosynthesis gene YUC4 in a coherent feed-forward loop, and this process is necessary to drive vascular regeneration. In the absence of this PLT-mediated regeneration response, leaf ground tissue cells can neither acquire the early vascular identity marker ATHB8, nor properly polarise auxin transporters to specify new venation paths. The PLT-CUC2 module is required for vascular regeneration, but is dispensable for midvein formation in leaves. We reveal the mechanisms of vascular regeneration in plants and distinguish between the wound-repair ability of the tissue and its formation during normal development.
Subject: Vascular regeneration
PLT
CUC2
Wound repair
Auxin
PIN1
Arabidopsis
ARABIDOPSIS
TISSUE
DIFFERENTIATION
PATTERN
GENES
MODEL
ACQUISITION
PHYLLOTAXIS
EXPRESSION
FRAMEWORK
1184 Genetics, developmental biology, physiology
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