An efficient auxin-inducible degron system with low basal degradation in human cells

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Li , S , Prasanna , X , Salo , V T , Vattulainen , I & Ikonen , E 2019 , ' An efficient auxin-inducible degron system with low basal degradation in human cells ' , Nature methods , vol. 16 , no. 9 , pp. 866-+ . https://doi.org/10.1038/s41592-019-0512-x

Title: An efficient auxin-inducible degron system with low basal degradation in human cells
Author: Li, Shiqian; Prasanna, Xavier; Salo, Veijo T.; Vattulainen, Ilpo; Ikonen, Elina
Other contributor: University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Materials Physics
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program
University of Helsinki, Department of Physics
University of Helsinki, STEMM - Stem Cells and Metabolism Research Program





Date: 2019-09
Language: eng
Number of pages: 7
Belongs to series: Nature methods
ISSN: 1548-7091
DOI: https://doi.org/10.1038/s41592-019-0512-x
URI: http://hdl.handle.net/10138/319568
Abstract: Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron minilAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
Subject: DEPLETION
PROTEINS
DRIVEN
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
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