An efficient auxin-inducible degron system with low basal degradation in human cells
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Li , S , Prasanna , X , Salo , V T , Vattulainen , I & Ikonen , E 2019 , ' An efficient auxin-inducible degron system with low basal degradation in human cells ' , Nature methods , vol. 16 , no. 9 , pp. 866-+ . https://doi.org/10.1038/s41592-019-0512-x
| Title: | An efficient auxin-inducible degron system with low basal degradation in human cells |
| Author: | Li, Shiqian; Prasanna, Xavier; Salo, Veijo T.; Vattulainen, Ilpo; Ikonen, Elina |
| Contributor organization: | STEMM - Stem Cells and Metabolism Research Program Department of Anatomy Medicum Materials Physics Department of Physics Lipid Trafficking Lab |
| Date: | 2019-09 |
| Language: | eng |
| Number of pages: | 7 |
| Belongs to series: | Nature methods |
| ISSN: | 1548-7091 |
| DOI: | https://doi.org/10.1038/s41592-019-0512-x |
| URI: | http://hdl.handle.net/10138/319568 |
| Abstract: | Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron minilAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes. |
| Subject: |
DEPLETION
PROTEINS DRIVEN 3111 Biomedicine 1182 Biochemistry, cell and molecular biology |
| Peer reviewed: | Yes |
| Rights: | unspecified |
| Usage restriction: | openAccess |
| Self-archived version: | publishedVersion |
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