Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction

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http://hdl.handle.net/10138/320979

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Huck , O , Mulhall , H , Rubin , G , Kizelnik , Z , Iyer , R , Perpich , J D , Haque , N , Cani , P D , de Vos , W M & Amar , S 2020 , ' Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction ' , Journal of Clinical Periodontology , vol. 47 , no. 2 , pp. 202-212 . https://doi.org/10.1111/jcpe.13214

Title: Akkermansia muciniphila reduces Porphyromonas gingivalis-induced inflammation and periodontal bone destruction
Author: Huck, Olivier; Mulhall, Hannah; Rubin, George; Kizelnik, Zev; Iyer, Radha; Perpich, John D; Haque, Nasreen; Cani, Patrice D; de Vos, Willem M; Amar, Salomon
Contributor: University of Helsinki, Medicum
Date: 2020-02
Language: eng
Number of pages: 11
Belongs to series: Journal of Clinical Periodontology
ISSN: 0303-6979
URI: http://hdl.handle.net/10138/320979
Abstract: Abstract Aim Akkermansia muciniphila is a beneficial gut commensal, whose anti-inflammatory properties have recently been demonstrated. This study aimed to evaluate the effect of A.muciniphila on Porphyromonas gingivalis elicited inflammation. Material and Methods In lean and obese mice, A.muciniphila was administered in P.gingivalis induced calvarial abcess and in experimental periodontitis model (EIP). Bone destruction and inflammation were evaluated by histomorphometric analysis. In vitro, A.muciniphila was co-cultured with P.gingivalis, growth and virulence factors expression were evaluated. Bone-marrow macrophages (BMM?) and gingival epithelial cells (TIGK) were exposed to both bacterial strains and the expression of inflammatory mediators, as well as tight junction markers was analyzed. Results In a model of calvarial infection, A.muciniphila decreased inflammatory cell infiltration and bone destruction. In EIP, treatment with A.muciniphila resulted in a decreased alveolar bone loss. In vitro, the addition of A.muciniphila to P.gingivalis infected BMM? increased anti-inflammatory IL-10 and decreased IL-12. Additionally, A.muciniphila exposure increases the expression of junctional integrity markers such as integrin-?1, E-cadherin and ZO-1 in TIGK cells. A.muciniphila co-culture with P.gingivalis reduced gingipains mRNA expression. Discussion This study demonstrated the protective effects of A.muciniphila administration and may open consideration to its use as an adjunctive therapeutic agent to periodontal treatment.
Subject: infection
inflammation
periodontitis
probiotic
THERAPY
MUCIN
INTEGRITY
MICE
313 Dentistry
11832 Microbiology and virology
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