A Na,K-ATPase-Fodrin-Actin Membrane Cytoskeleton Complex is Required for Endothelial Fenestra Biogenesis

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Ju , M , Ioannidou , S , Munro , P , Rämö , O , Vihinen , H , Jokitalo , E & Shima , D T 2020 , ' A Na,K-ATPase-Fodrin-Actin Membrane Cytoskeleton Complex is Required for Endothelial Fenestra Biogenesis ' , Cells , vol. 9 , no. 6 , 1387 . https://doi.org/10.3390/cells9061387

Title: A Na,K-ATPase-Fodrin-Actin Membrane Cytoskeleton Complex is Required for Endothelial Fenestra Biogenesis
Author: Ju, Meihua; Ioannidou, Sofia; Munro, Peter; Rämö, Olli; Vihinen, Helena; Jokitalo, Eija; Shima, David T.
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Electron Microscopy
University of Helsinki, Electron Microscopy
Date: 2020-06
Language: eng
Number of pages: 23
Belongs to series: Cells
ISSN: 2073-4409
URI: http://hdl.handle.net/10138/321030
Abstract: Fenestrae are transcellular plasma membrane pores that mediate blood-tissue exchange in specialised vascular endothelia. The composition and biogenesis of the fenestra remain enigmatic. We isolated and characterised the protein composition of large patches of fenestrated plasma membrane, termed sieve plates. Loss-of-function experiments demonstrated that two components of the sieve plate, moesin and annexin II, were positive and negative regulators of fenestra formation, respectively. Biochemical analyses showed that moesin is involved in the formation of an actin-fodrin submembrane cytoskeleton that was essential for fenestra formation. The link between the fodrin cytoskeleton and the plasma membrane involved the fenestral pore protein PV-1 and Na,K-ATPase, which is a key regulator of signalling during fenestra formation both in vitro and in vivo. These findings provide a conceptual framework for fenestra biogenesis, linking the dynamic changes in plasma membrane remodelling to the formation of a submembrane cytoskeletal signalling complex.
Subject: fenestrae
moesin
annexin II
actin
fodrin
Na
K-ATPase
submembrane cytoskeleton
NA+,K+-ATPASE ALPHA-SUBUNIT
ERYTHROCYTE-MEMBRANE
GROWTH-FACTOR
ACTIN CYTOSKELETON
NA+/K+-ATPASE
PROTEIN
SPECTRIN
PERMEABILITY
BINDING
OUABAIN
1182 Biochemistry, cell and molecular biology
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