1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi

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Virjamo , V , Fyhrquist , P , Koskinen , A , Lavola , A , Nissinen , K & Julkunen-Tiitto , R 2020 , ' 1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi ' , Molecules , vol. 25 , no. 19 , 4558 . https://doi.org/10.3390/molecules25194558

Title: 1,6-Dehydropinidine Is an Abundant Compound in Picea abies (Pinaceae) Sprouts and 1,6-Dehydropinidine Fraction Shows Antibacterial Activity against Streptococcus equi Subsp. equi
Author: Virjamo, Virpi; Fyhrquist, Pia; Koskinen, Akseli; Lavola, Anu; Nissinen, Katri; Julkunen-Tiitto, Riitta
Other contributor: University of Helsinki, Division of Pharmaceutical Biosciences
Date: 2020-10
Language: eng
Number of pages: 12
Belongs to series: Molecules
ISSN: 1420-3049
DOI: https://doi.org/10.3390/molecules25194558
URI: http://hdl.handle.net/10138/322010
Abstract: Knowledge about the defensive chemistry of coniferous trees has increased in recent years regarding a number of alkaloid compounds; in addition to phenolics and terpenes. Here, we show that Norway spruce (Picea abies (L.) H. Karst.), an important boreal zone tree species; accumulates 1,6-dehydropinidine (2-methyl-6-(2-propenyl)-1,6-piperideine) in its needles and bark. We reanalyzed previously published GC-MS data to obtain a full picture of 1,6-dehydropinidine in P. abies. 1,6-dehydropinidine appeared to especially accumulate in developing spring shoots. We used solid-phase partitioning to collect the alkaloid fraction of the sprouts and thin-layer chromatography to purify 1,6-dehydropinidine. The antibacterial properties of the 1,6-dehydropinidine fraction were tested using a broth microdilution method; with Streptococcus equi subsp. equi as a model organism. Based on our results 1,6-dehydropinidine is common in alkaloid extractions from P. abies (0.4 +/- 0.03 mg g(-1) dw in mature needles) and it is especially abundant in young spruce shoots (2.7 +/- 0.5 mg g(-1) dw). Moreover; 1,6-dehydropinidine extracted from P. abies sprouts showed mild antibacterial potential against Streptococcus equi subsp. equi (MIC 55 mu g mL(-1)). The antibacterial activity of a plant compound thought of as an intermediate rather than an end-product of biosynthesis calls for more detailed studies regarding the biological function of these coniferous alkaloids
Subject: pinaceae
norway spruce
Picea abies
Streptococcus equi
equine strangles
antibacterial
piperidine
alkaloid
PIPERIDINE ALKALOIDS
NORWAY SPRUCE
PINE PINUS
PHENOLIC-COMPOUNDS
SCOTS PINE
PONDEROSA
SUSCEPTIBILITY
SYLVESTRIS
CHEMISTRY
SEEDLINGS
1182 Biochemistry, cell and molecular biology
116 Chemical sciences
317 Pharmacy
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