Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila

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Gospodaryov , D , Strilbytska , O M , Semaniuk , U , Perkhulyn , N , Rovenko , B M , Yurkevych , I S , Barata , A G , Dick , T P , Lushchak , O & Jacobs , H T 2020 , ' Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila ' , Biogerontology , vol. 21 , no. 2 , pp. 155-171 . https://doi.org/10.1007/s10522-019-09849-8

Title: Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila
Author: Gospodaryov, Dmytro; Strilbytska, Olha M.; Semaniuk, Uliana; Perkhulyn, Natalia; Rovenko, Bohdana M.; Yurkevych, Ihor S.; Barata, Ana G.; Dick, Tobias P.; Lushchak, Oleh; Jacobs, Howard T.
Contributor: University of Helsinki, Institute of Biotechnology
Date: 2020-04
Language: eng
Number of pages: 17
Belongs to series: Biogerontology
ISSN: 1389-5729
URI: http://hdl.handle.net/10138/323385
Abstract: Mitochondrial alternative NADH dehydrogenase (aNDH) was found to extend lifespan when expressed in the fruit fly. We have found that fruit flies expressing aNDH from Ciona intestinalis (NDX) had 17-71% lifespan prolongation on media with different protein-tocarbohydrate ratios except NDX-expressing males that had 19% shorter lifespan than controls on a high protein diet. NDX-expressing flies were more resistant to organic xenobiotics, 2,4-dichlorophenoxyacetic acid and alloxan, and inorganic toxicant potassium iodate, and partially to sodium molybdate treatments. On the other hand, NDX-expressing flies were more sensitive to catechol and sodium chromate. Enzymatic analysis showed that NDX-expressing males had higher glucose 6-phosphate dehydrogenase activity, whilst both sexes showed increased glutathione S-transferase activity.
Subject: Mitochondria
Drosophila melanogaster
Reactive oxygen species
Antioxidant defence
Stress resistance
2,4-DICHLOROPHENOXYACETIC ACID 2,4-D
OXIDATIVE STRESS
ELECTRON-TRANSPORT
NRF2
EXPRESSION
LONGEVITY
NDI1
MELANOGASTER
ACTIVATION
MECHANISMS
1184 Genetics, developmental biology, physiology
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