AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions

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Harjumaki , R , Zhang , X , Nugroho , R W N , Farooq , M , Lou , Y-R , Yliperttula , M , Valle-Delgado , J J & Osterberg , M 2020 , ' AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions ' , ACS Applied Bio Materials , vol. 3 , no. 3 , pp. 1406-1417 . https://doi.org/10.1021/acsabm.9b01073

Title: AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell-Biomaterial Interactions
Author: Harjumaki, Riina; Zhang, Xue; Nugroho, Robertus Wahyu N.; Farooq, Muhammad; Lou, Yan-Ru; Yliperttula, Marjo; Valle-Delgado, Juan Jose; Osterberg, Monika
Contributor: University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Drug Research Program
Date: 2020
Language: eng
Number of pages: 12
Belongs to series: ACS Applied Bio Materials
ISSN: 2576-6422
URI: http://hdl.handle.net/10138/325112
Abstract: Transmembrane protein integrins play a key role in cell adhesion. Cell-biomaterial interactions are affected by integrin expression and conformation, which are actively controlled by cells. Although integrin structure and function have been studied in detail, quantitative analyses of integrin-mediated cell-biomaterial interactions are still scarce. Here, we have used atomic force spectroscopy to study how integrin distribution and activation (via intracellular mechanisms in living cells or by divalent cations) affect the interaction of human pluripotent stem cells (WA07) and human hepatocarcinoma cells (HepG2) with promising biomaterials.human recombinant laminin-521 (LN-521) and cellulose nanofibrils (CNF). Cell adhesion to LN-521-coated probes was remarkably influenced by cell viability, divalent cations, and integrin density in WA07 colonies, indicating that specific bonds between LN-521 and activated integrins play a significant role in the interactions between LN-521 and HepG2 and WA07 cells. In contrast, the interactions between CNF and cells were nonspecific and not influenced by cell viability or the presence of divalent cations. These results shed light on the underlying mechanisms of cell adhesion, with direct impact on cell culture and tissue engineering applications.
Subject: integrin
human pluripotent stem cells
human hepatocarcinoma cells
cellulose nanofibrils
laminin-521
atomic force microscope
force spectroscopy
colloidal probe microscopy
NANOFIBRILLAR CELLULOSE HYDROGEL
LIGAND-BINDING
ADHESION
QUANTIFICATION
FIBRONECTIN
CALIBRATION
MODULATION
MICROSCOPY
MORPHOLOGY
SURFACES
216 Materials engineering
221 Nano-technology
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